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D9510 Drop-out Base With Glucose and Agar (DO, Dropout, Powder)

Specifications
References
Grade
Molecular Biology Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C
Synthetic minimal media with glucose and agar

Nitrogen, carbon, trace element, and vitamin source for yeast growth used as a media base for yeast transformant selection. Supplement with the appropriate DO Mix for selection and propagation of yeast transformants. Glucose allows for the rapid growth of yeast with short doubling times.

All lots are quality control tested using the appropriate yeast strains and transformants.
• Convenient Powders • Useful for One-Hybrid and Two-Hybrid Systems • Competitively Priced...!
BSE-Free: All raw materials of animal origin are sourced from USDA-designated BSE-Free countries
Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates
Homogeneous: Proprietary blending technology ensures complete mixing of components
Appearance
White to off-white, homogeneous free flowing powder
Solubility
Colorless to light yellow, clear, complete after autoclaving
pH
5.7±0.5
Directions per Liter
Dissolve 43.7 grams per liter of distilled/deionized water, heating with stirring until completely solubilized. Adjust to desired pH. Dispense into appropriate containers, loosen caps and autoclave for 15 mins. at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as g/liter
Agar (1.7%)17
D-Glucose (2%)20
YNB (w/ AA & w/ AS) (0.67%)6.7
Total43.7g/L
References
1. Kaiser, C., Michaelis, S., Mitchell, A., Methods in Yeast Genetics, Cold Spring Harbor Laboratory Course Manual, Appendix A: 210, CSHL Press (1994).
USBio References
No references available
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