Drop-out Mix Synthetic Minus Histidine, Methionine, Cysteine, Adenine Rich w/o Yeast Nitrogen Base

Grade

Molecular Biology Grade

EU Commodity Code

38210000

Shipping Temp

Storage Temp

RT/4°C

Synthetic complete amino acid supplement minus Histidine, Methionine, Cysteine, Adenine Rich w/o Yeast Nitrogen Base

Drop-out Mix Complete (DOC) is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics.

Appearance

White, homogeneous, free flowing powder

Solubility

Colorless, clear, complete

As Reported

Directions per Liter (Broth)

Mix 1.8 grams of drop-out mix with 6.7g Y2025 Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS per liter of ddH2O. Adjust to desired pH. Sterilize with 0.2um membrane filter. Aliquot into sterile containers.

Directions per Liter (Plates)

Mix 1.8 grams of drop-out mix with 43.7g D9510-01 Drop-out Base (w/o aa) With Glucose and Agar per liter of ddH2O. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Storage and Stability

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Media Formulation

Each bottle is a homogeneous mixture of the following amino acids and nucleotide bases minus histidine, methionine and cysteine

Amino Acids

Alanine	2.0g
p-Aminobenzoic acid	0.2g
Arginine	2.0g
Asparagine	2.0g
Aspartic acid	2.0g
Cysteine	Absent
Glutamic acid	2.0g
Glutamine	2.0g
Glycine	2.0g
Histidine	Absent
Isoleucine	2.0g
Leucine	10.0g
Lysine	2.0g
Methionine	Absent
Phenylalanine	2.0g
Proline	2.0g
Serine	2.0g
Threonine	2.0g
Tryptophan	2.0g
Tyrosine	2.0g
Valine	2.0g

Nucleotide Bases

Adenine	2.0g
Uracil	2.0g

Vitamins

Inositol

2.0g

Use 1.8g/liter
100g makes ~50 liters

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D9537-19 Drop-out Mix Synthetic Minus Histidine, Methionine, Cysteine, Adenine Rich w/o Yeast Nitrogen Base (DO, Dropout, Powder)

Grade

EU Commodity Code

Shipping Temp

Storage Temp

Synthetic complete amino acid supplement minus Histidine, Methionine, Cysteine, Adenine Rich w/o Yeast Nitrogen Base

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