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D9811-06 Dulbecco’s MEM (DMEM) F-12 Complete Base w/ HEPES and Sodium Bicarbonate (Powder)

Specifications
References
Grade
Cell Culture Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C

Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

Appearance
Light pink to light orange, homogeneous, free flowing powder
Solubility
Orange to red, clear, complete
pH
As Reported
Endotoxin
≤1EU/ml
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Liter
Dissolve 16.77g in 800-900ml of ddH2O, stirring gently until completely solubilized. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as g/liter
Inorganic Salts
Calcium Chloride0.1166
Cupric Sulfate•5H2O 0.0000013
Ferric Nitrate•9H2O0.00005
Ferrous Sulfate•7H2O0.000417
Magnesium Chloride0.02864
Magnesium Sulfate0.04884
Potassium Chloride0.3118
Sodium Bicarbonate1.200
Sodium Chloride6.996
Sodium Phosphate Dibasic 0.07102
Sodium Phosphate Monobasic•H2O 0.0625
Zinc Sulfate•7H2O0.000432
Amino Acids
L-Alanine0.00445
L-Arginine•HCl0.1475
L-Asparagine•H2O 0.0075
L-Aspartic Acid0.00665
L-Cysteine•HCl•H2O 0.01756
L-Cystine•2HCl0.03129
L-Glutamic Acid 0.00735
L-Glutamine0.365
Glycine0.01875
L-Histidine HCl•H2O 0.03148
L-Isoleucine 0.05447
L-Leucine0.05905
L-Lysine•HCl0.09125
L-Methionine0.01724
L-Phenylalanine0.03548
L-Proline0.01725
L-Serine0.02625
L-Threonine0.05345
L-Tryptophan0.00902
L-Tyrosine•2Na•2H20 0.05579
L-Valine0.05285
Vitamins
Biotin0.0000035
Choline Chloride0.00898
Vitamin B120.00068
Folic Acid0.00265
i-inositol0.0126
Niacinamide0.00202
D-Pantothenic Acid•Ca0.00224
Pyridoxine•HCl 0.002
Riboflavin0.000219
Thiamine•HCl0.00217
Other
Thymidine0.000365
D-Glucose3.151
HEPES3.5745
Hypoxanthine (Na)0.00239
Linoleic Acid0.000042
Lipoic Acid0.000105
Phenol Red0.0081
Putrescine•2HCl 0.000081
Pyruvic Acid, Sodium0.055
Total:16.77g/Liter
References
1. Gibb AA, et al. Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes. Biochem J. 2017 Jul 13. pii: BCJ20170474. doi: 10.1042/BCJ20170474. 2. Biesemann N., et al. High throughput screening of mitochondrial bioenergetics in human differentiated myotubes identifies novel enhancers of muscle performance in aged mice. 2018. Sci Rep. 8(1):9408. doi: 10.1038/s41598-018-27614-8.
USBio References
1. Gibb AA, et al. Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes. Biochem J. 2017 Jul 13. pii: BCJ20170474. doi: 10.1042/BCJ20170474. 2. Biesemann N., et al. High throughput screening of mitochondrial bioenergetics in human differentiated myotubes identifies novel enhancers of muscle performance in aged mice. 2018. Sci Rep. 8(1):9408. doi: 10.1038/s41598-018-27614-8.
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