USBio Logo

D9812-05A Dulbecco’s MEM (DMEM) High Glucose w/Glutamax, w/o Phenol Red (Powder)

Specifications
References
Grade
Cell Culture Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C

Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

Appearance
Off-white to pale yellow homogenous, free flowing powder
Solubility
Colorless to pale yellow, clear, complete
pH
As Reported
Endotoxin
≤1EU/ml
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Liter Dissolve 12.89g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 3.7g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as mg/Liter
Inorganic Salts
Calcium Chloride•2H2O200
Ferric Nitrate•9H2O0.1
Magnesium Sulfate97.7
Potassium Chloride400
Sodium Chloride6400
Sodium Phosphate Monobasic, Anhy125
Amino Acids
L-Arginine•HCl84
L-Cystine62.57
Glutamax0.584
Glycine30
L-Histidine•HCl•H2O42
L-Isoleucine104.8
L-Leucine104.8
L-Lysine•HCl146.2
L-Methionine30
L-Phenylalanine66
L-Serine42
L-Threonine95.2
L-Tryptophan16
L-Tyrosine103.8
L-Valine94
Vitamins
Choline Chloride4
Folic Acid4
myo-Inositol7.2
Niacinamide4
D-Pantothenic Acid•Ca4
Pyridoxine•HCl4
Riboflavin0.4
Thiamine•HCl4
Other
D+Glucose4500
Pyruvic Acid, Sodium110
Phenol Red, SodiumAbsent
Total12.89g/L
References
1. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 2. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus. Virology 12(2): 185-196 (1960). 3. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro 6: 89. (1970). 4. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro 9(6): 468-9 (1974, May-Jun).
USBio References
No references available
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
  • Contact Us

    Visit our technical library or contact our support staff to answer your questions.

    Telephone:
    1-800-520-3011

    Library | Contact

    Distributors

    For customers outside of the United States, please use one of our many distributors.

    View Distributors

    Payment Methods

    We accept the following payment methods as well as pay-by-invoice.

    MasterCard Visa PayPal
    © 2023-2024 United States Biological - All Rights Reserved