Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.
Components shown as mg/Liter |
Calcium Chloride•2H2O | 200 |
Ferric Nitrate•9H2O | 0.1 |
Magnesium Sulfate | 97.7 |
Potassium Chloride | 400 |
Sodium Chloride | 6400 |
Sodium Phosphate Monobasic, Anhy | 125 |
L-Arginine•HCl | 84 |
L-Cystine | 62.57 |
Glutamax | 0.584 |
Glycine | 30 |
L-Histidine•HCl•H2O | 42 |
L-Isoleucine | 104.8 |
L-Leucine | 104.8 |
L-Lysine•HCl | 146.2 |
L-Methionine | 30 |
L-Phenylalanine | 66 |
L-Serine | 42 |
L-Threonine | 95.2 |
L-Tryptophan | 16 |
L-Tyrosine | 103.8 |
L-Valine | 94 |
Choline Chloride | 4 |
Folic Acid | 4 |
myo-Inositol | 7.2 |
Niacinamide | 4 |
D-Pantothenic Acid•Ca | 4 |
Pyridoxine•HCl | 4 |
Riboflavin | 0.4 |
Thiamine•HCl | 4 |
D+Glucose | 4500 |
Pyruvic Acid, Sodium | 110 |
Phenol Red, Sodium | Absent |
Total | 12.89g/L |
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