Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Conjugate
HRP
Specificity
Recognizes GRK 4, GRK 5 and GRK 6. Species Crossreactivity: Human, mouse and rat.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free BSA Free
Applications
Suitable for use in Western Blot, Immunoprecipitation, ELISA. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
General Freedman, N.J., et al., J. Biol. Chem. 272: 17734-11743, 1997. Pitcher, J.A., et al., J. Biol. Chem. 271: 24907-24913, 1996. Nagayama, Y., et al., J. Biol. Chem. 271: 10143-10148, 1996. Application Oppermann, M., et al., Proc. Natl. Acad. Sci. USA 93: 7649-7654, 1996.
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