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H6099-95B-APC HLA Class 2 Antigen DR (APC)

Specifications
Grade
Affinity Purified
Purity
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Conjugate
APC
Specificity
Recognizes the human HLA-DR cell surface antigen. Recognizes a determinant that is dependent upon the association of both the a and b chains of HLA-DR.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: Use 10ul to label 10e6 cells or 100ul whole blood. Immunohistochemistry: Frozen. Not tested on paraffin tissues. Optimal working dilutions to be determined by researcher.
Recommended Secondary Reagents
F(ab')2 Rabbit anti-mouse IgG labled with FITC or RPE Immunohistochemistry: F(ab')2 Rabbit anti-mouse IgG labled with HRP
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
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