Supplied as a liquid in 30mM triethanolamine, pH 7.2, 5mM magnesium chloride, 0.1mM zinc|chloride, 1% BSA, Protease/IgG free, 0.05% sodium azide. Labeled with Alkaline Phosphatase (AP).
Conjugate
AP
Specificity
Recognizes the heavy chains on chicken IgG and with the light chains on all chicken immunoglobulins based on IEP.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:500-1:2000 Western Blot: 1:500-1:2000 Immunohistochemistry: 1:20-1:2000 Immunocytochemistry: 1:50-1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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