Supplied as a lyophilized powder from 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2 1% BSA (Protease/IgG free), 0.05% sodium azide. Labeled with DyLight®488. Reconstitute with 1.1ml sterile ddH2O.
Conjugate
DyLight®488
Specificity
Recognizes the heavy chains on chicken IgG and with the light chains on all chicken immunoglobulins based on IEP.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Applications
Suitable for use in Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: 1:20-1:2000 Immunocytochemistry: 1:50-1:5000 Optimal dilutions to be determined by the researcher.
Absorption
493nm
Emission
518nm
Fluorochrome/Protein Ratio
1-5 Moles DyLight™ 488 per Mole
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at 4°C. Reconstitute with sterile ddH2O. Do not freeze. Aliquot to avoid repeated freezing and thawing. Store at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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