Supplied as a lyophilized powder in 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA, Protease/IgG free, 0.1% Kathon CG. Labeled with horseradish peroxidase (HRP).
Conjugate
HRP
Specificity
Recognizes heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on IEP.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
-20°C
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:10,000-1:50,000 Western Blot: 1:10,000-1:50,000 Immunohistochemistry: 1:500-1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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