Purified by immunoaffinity chromatography. ~90% by SDS-PAGE. May contain small amounts of intact IgG.
Form
Supplied as a liquid in 30mM triethanolamine, pH 7.2, 5mM magnesium chloride, 0.1mM zinc chloride, 1% BSA (Protease/IgG free), 0.05% sdium azide. Labeled with Alkaline Phosphatase (AP).
Conjugate
AP
Specificity
Recognizes heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on IEP.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 1:500-1:8000 Western Blot: 1:500-1:8000 Immunohistochemistry: 1:500-1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
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