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K0199-17P KRT14, Recombinant, Human, aa1-472, His-Tag (Keratin, Type I Cytoskeletal 14, Cytokeratin-14, CK-14, Keratin-14, K14)

Specifications
References
Grade
Purified
Purity
≥90% (SDS-PAGE)
Form
Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 10% glycerol, 0.4M urea.
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

KRT14, is a member of the cytokeratin subfamily of intermediate filament proteins (IFPs). It is unique in that it is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. It functions to regulate cell growth and size through its interactions with the adaptor protein 14-3-3 sigma to mediate protein synthesis.

Source
Recombinant corresponding to aa1-472 from human KRT14, fused to His-tag at N-terminal expressed in E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MTTCSRQFTS SSSMKGSCGI GGGIGGGSSR ISSVLAGGSC RAPSTYGGGL SVSSSRFSSG GAYGLGGGYG GGFSSSSSSF GSGFGGGYGG GLGTGLGGGF GGGFAGGDGL LVGSEKVTMQ NLNDRLASYL DKVRALEEAN ADLEVKIRDW YQRQRPAEIK DYSPYFKTIE DLRNKILTAT VDNANVLLQI DNARLAADDF RTKYETELNL RMSVEADING LRRVLDELTL ARADLEMQIE SLKEELAYLK KNHEEEMNAL RGQVGGDVNV EMDAAPGVDL SRILNEMRDQ YEKMAEKNRK DAEEWFFTKT EELNREVATN SELVQSGKSE ISELRRTMQN LEIELQSQLS MKASLENSLE ETKGRYCMQL AQIQEMIGSV EEQLAQLRCE MEQQNQEYKI LLDVKTRLEQ EIATYRRLLE GEDAHLSSSQ FSSGSQSSRD VTSSSRQIRT KVMDVHDGKV VSTHEQVLRT KN
Molecular Weight
~53.8kD
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥90% (SDS-PAGE)
Concentration
~1mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 10% glycerol, 0.4M urea.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Romano RA. et al. (2007) J Invest Dermatol. 127:1175-1186.(2) Coulombe PA. et al. (1991) Cell. 66 (6): 1301
USBio References
No references available
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