LB Broth media formulations have been industry standards for the cultivation of Escherichia coli since the 1950’s (1,2,3). These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins (4,5). The media are nutrient-rich formulations which provide peptides and peptones, vitamins, and trace elements. The three formulations (Lennox, Miller and Luria) differ in the amount of sodium chloride, thus providing selection of the appropriate osmotic conditions for the particular bacterial strain and desired culture conditions. The low salt formulations, Lennox and Luria, are ideal for cultures requiring salt-sensitive antibiotics such as Zeocin™.

Principles of the Procedure: Peptides and peptones are provided by Soy Peptone. Vitamins (including B vitamins) and certain trace elements are provided by Yeast Extract. Sodium ions for transport and osmotic balance are provided by sodium chloride.

Light tan, homogeneous, free flowing powder

Pale yellow to yellow, clear, complete

As Reported

Terrific Broth is a richer media that would be suitable if higher cell densities and higher yields for plasmids or protein purifications are necessary. T2810: Terrific Broth (Powder) T2810-05: Terrific Broth, Complete with Carbon Source (Powder) T2810-10: Terrific Broth, Modified for Genomics (Powder) T2810-11: Terrific Broth, Modified for Fermentation, non-animal (Powder)

L1500: LB Agar Lennox (Powder) L1505: LB Broth Lennox (Powder) L1510: LB Top Agar Lennox (Powder) P9102-40H: Protein Expression Media, LB Broth Lennox (Powder) pEX™ P9102-40H1: Protein Expression Media, LB Broth Lennox (Liquid) pEX™

L1515: LB Agar Miller (Powder) L1520: LB Broth Miller (Powder) L1525: LB Top Agar Miller (Powder) P9102-40G: Protein Expression Media, LB Broth Miller (Powder) pEX™ P9102-40G1: Protein Expression Media, LB Broth Miller (Liquid) pEX™

L1530: L-Broth (Luria Broth) (Powder)

Dissolve 20g in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.