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M2162-06-L Mammary Epithelial Basal Medium MCDB 170 w/o Glutamine, Glucose (Liquid)

Specifications
References
Grade
Cell Culture Grade
Form
Light yellow, clear, complete liquid.
Shipping Temp
Blue Ice
Storage Temp
4°C

Basal nutrient medium used for the growth of mammary epithelial cells. Supplement with growth factors or fetal calf serum (0.2-2.0% dialyzed).

Suggested Supplements: BPE 0.4%, Epidermal Growth Factor 10ng/ml, Insulin 5ug/ml, Hydrocortisone 0.5ug/ml, Isoproterenol 10uM, Transferrin 5ug/ml, Ethanolamine 0.1mM, Phosphoethanolamine 0.1mM, Amphotericin B 50ng/ml, Gentamicin 50ug/ml
pH
As Reported
Endotoxin
≤1EU/ml
Storage and Stability
Store at 4°C. Stable for 6 months after receipt.
Media Formulation
Components shown asmg/Liter
Inorganic Salts
Ammonium Metavandate0.000585
Ammonium Molybdate•4H2O0.001236
Calcium Chloride222.00
Cupric Sulfate0.000160
Ferrous Sulfate•7H2O1.39
Magnesium Sulfate180.57
Manganese Sulfate0.000121
Nickel Chloride•6H2O0.000001189
Potassium Chloride186.40
Potassium Phosphate Monobasic68.05
Sodium Chloride7014.00
Sodium Metasilicate•9H2O0.1421
Sodium Selenite0.005188
Stannous Chloride•2H2O0.000001128
Zinc Sulfate•7H2O0.0897736
Amino Acids
L-Alanine8.909
L-Arginine52.27
L-Asparagine132.10
L-Aspartic Acid13.31
L-Cysteine•HCl11.029
L-Glutamic Acid14.71
L-GlutamineAbsent
Glycine7.507
L-Histidine15.52
L-Isoleucine13.12
L-Leucine39.36
L-Lysine•HCl36.54
L-Methionine4.476
L-Phenylalanine4.956
L-Proline5.755
L-Serine31.53
L-Threonine35.73
L-Tryptophan6.126
L-Tyrosine9.06
L-Valine35.16
Vitamins
Biotin0.007329
Choline Chloride13.96
Vitamin B120.13550
Folinic Acid, Calcium0.005115
Myo-Inositol18.02
Nicotinamide6.105
D-Pantothenic Acid•Ca0.2383
Pyridoxine•HCl0.06168
Riboflavin0.11292
Thiamine•HCl0.3373
Other Components
Adenine0.1351
Thymidine0.07266
D-GlucoseAbsent
HEPES Buffer7149.00
Lipoic Acid0.002063
Phenol Red, Sodium1.242
Putrescine•2HCl0.000161
Sodium Pyruvate110.00
Total:15.45g/liter
References
Partanen, J.I. et al., (2007) PNAS 104:14694-14699. 1. Hammond, S. L. et al. Proc. Natl. Acad. Sci. USA Vol. 81; 5435-5439 (1984). 2. Peehl, D.M. and Ham, R.G., In Vitro: 16:526 (1980). 3. Bettger, W.J. et al., Proc. Natl, Acad. Soc., USA:78:, 5588-5592 (1981). 4. Stampfer, M.R. and Bartley, J. C. PNAS 82; 2394-2398 (1985)
USBio References
No references available
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