Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle's Basal Medium (BME). Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks' or Eagles' salts has broadened the usefulness of this medium.
Appearance
Light pink to orange, homogeneous, free flowing powder
Solubility
Orange, clear, complete
Directions per Liter
Dissolve 9.53g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2.2g sodium bicarbonate and stir until dissolved. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Inorganic Salts
| Calcium Chloride | 200.00 |
| Magnesium Sulfate | 97.70 |
| Potassium Chloride | 400.00 |
| Sodium Chloride | 6800.00 |
| Sodium Phosphate Monobasic•H2O | 140.00 |
Amino Acids
| L-Arginine•HCl | 127.00 |
| L-Cystine•2HCl | 32.28 |
| L-Glutamine | 292.00 |
| L-Histidine•HCl•H2O | 41.90 |
| L-Isoleucine | 52.00 |
| L-Leucine | 52.00 |
| L-Lysine•HCl | 73.00 |
| L-Methionine | 15.00 |
| L-Phenylalanine | 32.00 |
| L-Threonine | 48.00 |
| L-Tryptophan | 10.00 |
| L-Tyrosine•2Na•2H2O | 52.00 |
| L-Valine | 46.00 |
Vitamins
| Choline Chloride | 1.00 |
| Folic Acid | 1.00 |
| myo-Inositol | 2.00 |
| Nicotinamide | 1.00 |
| D-Pantothenic Acid•Ca | 1.00 |
| Pyridoxal•HCl | 1.00 |
| Riboflavin | 0.10 |
| Thiamine•HCl | 1.00 |
Other
| D-Glucose | 1000.00 |
| Phenol Red, Sodium | 10.00 |
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