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M5000 Mueller Hinton Medium (Agar) (Powder)

Specifications
References
Grade
Microbiological Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C
Mueller Hinton Medium

Mueller Hinton Agar is used in antimicrobial susceptibility testing by the disk diffusion method. This formula conforms to National Committee for Clinical Laboratory Standards (NCCLS).

Quality Control
Tested according to USB Standard Test Methods and NCCLS M6-A. Testing was performed in compliance with ISO/TS 11133-1 and BS EN 12322:1999 + A1:2001 standards. This product met or exceeded established NCCLS performance specifications.
Appearance
Beige, homogenous, free-flowing powder
Solubility
Light to medium yellow, clear, complete after autoclaving.
pH
7.3 ± 0.1
Directions per Liter
Dissolve 38 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Directions per Liter
Dissolve 38grams in 1L of ddH2O stirring gently with heating until completely solubilized. Boil for 1 minute. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Formulation for 1 Liter
Components shown as g/liter
Beef Extract.2
Acid Hydrolysate of Casein.17.5 Starch
Agar17
Total:38.0g/L
References
1. National Committee for Clinical Laboratory Standards. 1997. Performance standards for antimicrobial disk susceptibility tests. Approved standard M2-A6. National Committee for Clinical Laboratory Standards, Wayne, PA. 2. Mueller, J. H., and J. Hinton. 1941. A protein-free medium for primary isolation of gonococcus and meningococcus. Proc. Soc. Exp. Biol. Med. 48:3330-333. 3. Gordon and Hine. 1916. Br. Med. J. 678. 4. Bauer, A. L., W. M. M. Kirby, J. C. Sherris, and M. Turck. 1966. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45:493-496. 5. World Health Organization. 1961. Standardization of methods for conducting microbic sensitivity tests. Technical Report Series No. 210, Geneva. 6. Food and Drug Administration. Bacteriological analytical manual, 8th ed., AOAC International, Gaithersburg, MD. 7. Wood, G. L., and J. A. Washington. 1995. Antibacterial susceptibility tests: dilution and disk diffusion methods, p. 1327-1341. In Murray, P.R., E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (eds.). Manual of clinical microbiology, 6th ed. American Society for Microbiology, Washington, D.C. 8. National Committee for Clinical Laboratory Standards. 1996. Protocols for evaluating dehydrated; App. Standard. Wayne PA. 9. National Committee for Clinical Laboratory Standards. 1999. M100-S9. Performance Standards for Antimicrobial Susceptibility Testing; Ninth Informational Supplement. Wayne, PA. 10. Isenberg, H. D. (ed.). 1992. Clinical microbiology procedures handbook, vol. 1, American Society for Microbiology, Washington, D.C.
USBio References
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