Light tan to pale yellow, homogeneous, free flowing powder

Light tan, incomplete

Light tan, clear, complete

As Reported

Place the sterile N1000 solution into a 58°C water bath. N1000 solution needs to completely cool to the desired temperature (58°C). If the medium is not properly cooled when the CaCl2, MgSO4 and K2HPO4 are added, crystals will form in the agar.

a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solution b)Sterile 1M CaCl2: 1ml/liter N1000 solution c) Sterile 1M MgSO4: 1ml/liter N1000 solution Allow the solution to mix for five minutes before starting to pour plates to ensure that a homogeneous mixture has been achieved. *1M Phosphate buffer is prepared by dissolving 108.3g KH2PO4 and 35.6g K2HPO4 in ddH2O. QS to 1L).

Dissolve 23.005 grams in 973ml of ddH2O. Heat with stirring until the agar dissolves (cholesterol will typically remain undissolved). Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Place the sterile N1000 solution into a 58°C water bath. N1000 solution needs to completely cool to the desired temperature (58°C). If the media is not properly cooled when the CaCl2, MgSO4 and K2PO4 are added, crystals will form in the agar.

a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solution b)Sterile 1M CaCl2: 1ml/liter N1000 solution c) Sterile 1M MgSO4: 1ml/liter N1000 solution Allow the solution to mix for five minutes before starting to pour plates to ensure that a homogeneous mixture has been achieved. *1M Phosphate buffer is prepared by dissolving 108.3g KH2PO4 and 35.6g K2HPO4 in ddH2O. QS to 1L.

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.