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N1010 Nematode Growth Medium, 100X (Trace Metals Mix) (Powder)

Specifications
Grade
Molecular Biology Grade
EU Commodity Code
38210000
UN DOT Shipping
UN3077 PGIII
Shipping Temp
RT
Storage Temp
RT/4°C

Trace metals conveniently formulated for use in S-basal media

Appearance
Light green, homogeneous, free flowing powder
Solubility (1X)
Colorless to light yellow, clear, complete
pH (1X)
As Reported
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards
 All products should be handled by qualified personnel only, trained in laboratory procedures.
Directions per Liter for 100X
Dissolve 3.06 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L.
Directions for 500ml S-basal media
2.9g sodium chloride 25ml 1M KH2PO4 475ml DDW 0.5ml cholesterol prepared at 5mg/ml
3. Add the following
5.0ml trace metals solution 1.5ml 1M magnesium sulfate 3.0ml 0.5M calcium chloride. 2. Autoclave. Keep dark (wrap in aluminum foil). 5ml 1M potassium citrate, pH 6.0 5ml100X Pen/Strep/Neo 5ml Nystatin.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Formulation shown as g/liter for 100X
Cupric Sulfate Pentahydrate0.024
Disodium EDTA1.860
Ferric Sulfate Heptahydrate0.692
Manganese Chloride Tetrahydrate0.196
Zinc Sulfate Heptahydrate0.288
Total:3.06g/Liter
153g makes 50 liters
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