Appearance
Orange to pink, homogeneous, free flowing powder
Solubility
Orange, clear, complete
Directions per Liter
Dissolve 9.61g in 800-900ml of ddH2O, stirring gently until completely solubilized. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Components shown as mg/liter |
Inorganic Salts
| Calcium Chloride | 200.0 |
| Ferric Nitrate•9H2O | 0.100 |
| Magnesium Chloride | 77.3 |
| Potassium Chloride | 400.0 |
| Sodium Bicarbonate | 2200 |
| Sodium Chloride | 3000 |
| Sodium Phosphate Monobasic | 125.0 |
| Zinc Sulfate•7H2O | 0.194 |
Amino Acids
| L-Alanine | 2.000 |
| L-Arginine•HCl | 84.00 |
| L-Asparagine•H2O | 0.830 |
| L-Cysteine | 31.50 |
| L-Glutamine | Absent |
| Glycine | 30.00 |
| L-Histidine•HCl•H2O | 42.00 |
| L-Isoleucine | 105.00 |
| L-Leucine | 105.00 |
| L-Lysine•HCI | 146.0 |
| L-Methionine | 30.00 |
| L-Phenylalanine | 66.00 |
| L-Proline | 7.760 |
| L-Serine | 42.00 |
| L-Threonine | 95.00 |
| L-Tryptophan | 16.00 |
| L-Tyrosine | 72.00 |
| L-Valine | 94.00 |
Vitamins
| Choline Chloride | 4.00 |
| Vitamin B12 | 0.0068 |
| Folic Acid | 4.00 |
| Inositol | 7.20 |
| Niacinamide | 4.00 |
| D-Pantothenic Acid•Na | 4.00 |
| Pyridoxal•HCl | 4.00 |
| Riboflavin | 0.40 |
| Thiamine•HCI | 4.00 |
Other
| D-Glucose | Absent |
| HEPES | 2600 |
| Phenol Red | 8.10 |
| Sodium Pyruvate | Absent |
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