Media base for the induction of PL promoter based constructs in E.coli. Requires carbohydrate source (glucose) to support growth.
Appearance
Homogeneous and free flowing powder
Solubility
Clear, colorless and complete
Directions per Liter Dissolve 12.6 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage Store at 0-5ºC (store unopened media at 2-30°C). Opened bottles should be capped tightly and kept in a dark, low humidity environment.
Components shown as g/liter |
Sodium Phosphate Dibasic | 6.0 |
Potassium Phosphate Monobasic | 3.0 |
Casamino Acids | 2.0 |
Ammonium Chloride | 1.0 |
Sodium Chloride | 0.5 |
Magnesium Chloride | 0.095 |