RPMI 1640 is used for the culture of human normal and neoplastic leukocytes. RPMI 1640 was developed by Moore et. al. at Roswell Park Memorial Institute.
Components shown in mg/L |
Calcium Nitrate•4H2O | 100 |
Magnesium Sulfate | 48.8 |
Potassium Chloride | 400 |
Sodium Chloride | 6000 |
Sodium Phosphate Dibasic | 800.7 |
L-Arginine | 200 |
L-Asparagine | 50 |
L-Aspartic Acid | 20 |
L-Cystine | Absent |
L-Glutamic Acid | 20 |
L-Glutamine | Absent |
Glycine | 10 |
L-Histidine | 15 |
Hydroxy-L-proline | 20 |
L-Isoleucine | 50 |
L-Leucine | 50 |
L-Lysine•HCl | 40 |
L-Methionine | Absent |
L-Phenylalanine | 15 |
L-Proline | 20 |
L-Serine | 30 |
L-Threonine | 20 |
L-Tryptophan | 5 |
L-Tyrosine•2Na•2H2O | 28.83 |
L-Valine | 20 |
p-Aminobenzoic Acid | 1 |
D-Biotin | 0.2 |
Choline Chloride | 3 |
Cyanocobalamin | 0.005 |
Folic Acid | 1 |
i-Inositol | 35 |
Nicotinamide | 1 |
D-Pantothenic Acid, Ca | 0.25 |
Pyridoxine•HCl | 1 |
Riboflavin | 0.2 |
Thiamine•HCl | 1 |
D-Glucose | 2000 |
Glutathione,Reduced | 1 |
Phenol Red, Sodium | 5.3 |
Total: | 10g/L |
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