A USP medium for use in performing total combined yeast and mold counts (microbial limit tests).
Appearance
Light beige, homogenous, free flowing powder
Solubility
Yellow to light amber, clear, complete after autoclaving
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Liter
Dissolve 65 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Agar | 15.0 |
Dextrose | 40.0 |
Casein Digest Peptone | 10.0 |