Schneider’s Insect medium was developed and later modified by Dr. Schneider to support the growth and development of excised imaginal disks from the fruitfly, Drosophila melanogaster. The medium was later used to establish cell lines from Drosophila embryos. When supplemented with 5-20% heat-inactivated fetal bovine serum, Schneiders medium has been found to support the rapid growth of both primary and established cultures of cells derived from Drosophila spp. and several other dipterans.
| Magnesium Sulfate | 1.807221 |
| Potassium Chloride | 1.6 |
| Sodium Bicarbonate | Absent |
| Potassium Phosphate Monobasic | 0.45 |
| Sodium Chloride | 2.1 |
| Sodium Phosphate Dibasic | 0.7 |
| ß-Alanine | Absent |
| L-Arginine | Absent |
| L-Cystine•2HCl | Absent |
| L-Cysteine | Absent |
| L-Glutamic Acid | Absent |
| L-Glutamine | 1.8 |
| Glycine | Absent |
| L-Histidine | Absent |
| L-Isoleucine | Absent |
| L-Leucine | Absent |
| L-Lysine | Absent |
| L-Methionine | Absent |
| L-Proline | Absent |
| L-Serine | Absent |
| L-Threonine | Absent |
| L-Tryptophan | Absent |
| L-Tyrosine•2Na•2H2O | Absent |
| L-Valine | Absent |
| Fumaric Acid | 0.06 |
| D(+)-Glucose | 2 |
| a-Ketoglutaric Acid | 0.35 |
| L(-)-Malic Acid | 0.6 |
| Succinic Acid | 0.06 |
| D(+)-Trehalose | 2 |
| Yeast Extract | Absent |
| Total: | 13.53g/L |
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