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S0100-01 Schneider’s Insect Medium w/L-Glutamine w/o Calcium Chloride, Sodium Bicarbonate, Amino Acids, Yeast (Powder)

Specifications
References
Grade
Cell Culture Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C

Schneider’s Insect medium was developed and later modified by Dr. Schneider to support the growth and development of excised imaginal disks from the fruitfly, Drosophila melanogaster. The medium was later used to establish cell lines from Drosophila embryos. When supplemented with 5-20% heat-inactivated fetal bovine serum, Schneiders medium has been found to support the rapid growth of both primary and established cultures of cells derived from Drosophila spp. and several other dipterans.

Appearance
White homogenous, free flowing powder
Solubility
Colorless, clear, complete
pH
3.5±0.5
Directions per Liter
Dissolve 13.53g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add 0.4g/L sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown in g/L
Inorganic Salts
Magnesium Sulfate 1.807221
Potassium Chloride1.6
Sodium BicarbonateAbsent
Potassium Phosphate Monobasic0.45
Sodium Chloride2.1
Sodium Phosphate Dibasic0.7
Amino Acids
ß-AlanineAbsent
L-ArginineAbsent
L-Cystine•2HClAbsent
L-CysteineAbsent
L-Glutamic AcidAbsent
L-Glutamine1.8
GlycineAbsent
L-HistidineAbsent
L-IsoleucineAbsent
L-LeucineAbsent
L-LysineAbsent
L-MethionineAbsent
L-ProlineAbsent
L-SerineAbsent
L-ThreonineAbsent
L-TryptophanAbsent
L-Tyrosine•2Na•2H2OAbsent
L-ValineAbsent
Other
Fumaric Acid 0.06
D(+)-Glucose2
a-Ketoglutaric Acid0.35
L(-)-Malic Acid 0.6
Succinic Acid0.06
D(+)-Trehalose2
Yeast ExtractAbsent
Total: 13.53g/L
References
1. Kim W, et al. Spatial Activation of TORC1 Is Regulated by Hedgehog and E2F1 Signaling in the Drosophila Eye. 2017. Dev Cell. 42(4):363-375.e4. doi: 10.1016/j.devcel.2017.07.020.
USBio References
1. Kim W, et al. Spatial Activation of TORC1 Is Regulated by Hedgehog and E2F1 Signaling in the Drosophila Eye. 2017. Dev Cell. 42(4):363-375.e4. doi: 10.1016/j.devcel.2017.07.020.
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