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T2810-05 Terrific Broth, Complete with Carbon Source (Powder)

Specifications
References
Grade
Molecular Biology Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C
Yeast Extract, Tryptone, Sodium Pyruvate, Potassium phosphate dibasic, Potassium phosphate monobasic

Terrific Broth is a highly enriched bacteriological medium specifically designed by Tartof and Hobbs to increase the yield of plasmid DNA derived from suspension cultures of E. coli due in part to the increased buffering capacity derived from using mono and dibasic phosphates. T2810-05 is specially formulated with pyruvic acid as an alternate carbon source, eliminating the need to add glycerol. Provides the same high yield of plasmid DNA as traditional Terrific Broth.

BSE-Free: All raw materials of animal origin are sourced from USDA-designated BSE-Free countries.
Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates.
Homogeneous: Proprietary blending technology ensures complete mixing of components.
Appearance
Solubility
pH
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Liter
Dissolve 51.6 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). If precipitate forms after autoclaving, medium may be filter-sterilized using a 0.2 micron filter.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as g/liter
Potassium phosphate dibasic9.4
Potassium phosphate monobasic2.2
SodiumPyruvate4.0
Tryptone12.0
YeastExtract24.0
Total:51.6g/liter
References
Tartof and Hobbs (1987)
USBio References
No references available
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