Terrific Broth is a highly enriched bacteriological medium specifically designed by Tartof and Hobbs to increase the yield of plasmid DNA derived from suspension cultures of E. coli due in part to the increased buffering capacity derived from using mono and dibasic phosphates. Non-animal sourced Tryptone: Utilizes a plant-based tryptone manufactured by a controlled enzymatic hydrolysis of plant proteins containing a mixture of peptides and free amino acids which provide a source of organic nitrogen and growth factors.
Components shown as g/liter |
Potassium phosphate dibasic | 9.4 |
Potassium phosphate monobasic | 2.2 |
Tryptone (Animal Protein Free) | 12 |
Yeast Extract | 24 |
Total: | 47.6g/Liter |
500g makes 10.5 liters |
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