Thermophilus Vitamin-Mineral Stock 100X

Grade

Molecular Biology Grade

EU Commodity Code

38210000

Shipping Temp

Storage Temp

4°C

Used to prepare Thermophilus Growth Media

Castenholz TYE media is designed to support the growth of Thermophilus as it is too nutrient-poor to support many other bacterial life.

Appearance

Yellow, homogenous, free-flowing powder

Solubility

Yellow to amber, clear, complete

2.0±0.3

Directions per Liter Thermophilus Vitamin-Mineral Stock 100X (Powder), T4800

Dissolve 4.052g per liter of ddH2O, stirring gently until completely solubilized. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Note: The 100X liquid medium forms precipitates at pH values greater than 5. If higher pH is required, it is advisable to prepare a 2X solution from the stock 100X solution and adjust this solution to the desired pH. Filter-sterilize using a 0.22 micron membrane filter. Adjust the volume accordingly for use with Thermophilus Growth Medium Base Broth.

Storage and Stability

Store powdered media at 4°C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Media Formulation

Components shown as g/liter for 100X

Minerals

Boric Acid	0.05
Calcium Chloride	0.66
Cobalt Nitrate	0.005
Copper Sulfate	0.015
Ferric Chloride	0.50
Magnesium Chloride	2.50
Manganese Sulfate	0.20
Nickel Chloride	0.002
Sodium Molybdate	0.0025
Zinc Sulfate	0.05

Vitamins

p-Aminobenzoate	0.008
Biotin	0.001
Choline Bromide	0.005
Cyanocobalamin	0.00004
Folic Acid	0.001
myo-Inositol	0.002
Nicotinamide	0.008
Monosodium Orotic Acid	0.002
D-Pantothenic Acid•Ca	0.008
Pyridoxine Hydrochloride	0.008
Riboflavin	0.004
Spermidine	0.010
Thiamine Hydrochloride	0.010

Total:

4.052g/liter

Directions per Liter Thermophilus Vitamin-Mineral Stock 100X (Powder), T4800

Dissolve 4.05grams per liter of distilled/deionized (DDI) water. Heat with stirring until completely solubilized. Sterilize with 0.2um membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Thermophilus Growth Medium Base Broth

Peptone	5g
Yeast Extract	2g
Sodium Chloride	2g
Total:	9g/L

Directions per Liter Thermophilus Growth Medium Base Broth

Dissolve 9 grams of Thermophilus Growth Medium Base per liter of distilled/deionized (DDI) water. Heat with stirring until completely solubilized. Dispense into appropriate containers. Loosen caps or cover with foil. Autoclave for 15 minutes at 121ºC (15psi). After cooling, asceptically, add 10ml of prepared, sterile Thermophilus Vitamin-Mineral Stock 100X (T4800) to sterile Thermophilus Growth Medium Base Broth.