TNM-FH Insect Medium w/L-Glutamine w/o CaCl2, MgCl2, MgSO4

Grade

Cell Culture Grade

EU Commodity Code

38210000

Shipping Temp

Storage Temp

RT/4°C

A number of modifications of Grace's medium were developed in several laboratories in the late 1960's and 1970's. Many of these modifications were developed by Dr. W.F. Hink for the growth of cells derived from the cabbage looper, Tricoplusia ni. This formula represents the modification known as TNM-FH. When properly supplemented, TNM-FH Insect Medium supports a number of cell lines from a variety of lepidopteran species. It is ideally suited for growth, infection, and protein expression of insect cell lines. This medium may be used for suspension or monolayer cultures. This medium is generally supplemented with serum.

Appearance

Solubility

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Directions per Liter

Dissolve 47.8g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Media Formulation

Components shown as g/L

Inorganic Salts

Potassium Chloride	2.24
Sodium Phosphate Monobasic	0.876923

Amino Acids

L-Alanine	0.225
ß-Alanine	0.20
L-Arginine•HCl	0.70
L-Asparagine	0.35
L-Aspartic Acid	0.35
L-Cystine•2HCl	0.025
L-Glutamic Acid	0.60
L-Glutamine	0.60
Glycine	0.65
L-Histidine	2.50
L-Isoleucine	0.05
L-Leucine	0.075
L-Lysine•HCl	0.625
L-Methionine	0.05
L-Phenylalanine	0.15
L-Proline	0.35
DL-Serine	1.10
L-Threonine	0.175
L-Tryptophan	0.10
L-Tyrosine•2Na	0.07202
L-Valine	0.10

Vitamins

p-Aminobenzoic Acid	0.00002
D-Biotin	0.00001
Choline Chloride	0.0002
Folic Acid	0.00002
Myo-Inositol	0.00002
Niacin	0.00002
D-Pantothenic Acid•Ca	0.00002
Pyridoxine•HCl	0.00002
Riboflavin	0.00002
Thiamine	0.00002

Other

D(-)Fructose	0.40
Fumaric Acid	0.055
D(+)-Glucose	0.70
a-Ketoglutaric Acid	0.37
Lactalbumin Hydrolysate	3.3333
L(-)-Malic Acid	0.67
Succinic Acid	0.06
Sucrose	26.68
Yeast Extract	3.3333

Total:

47.8g/L

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T7000-05 TNM-FH Insect Medium w/L-Glutamine w/o CaCl2, MgCl2, MgSO4 (Powder)

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