Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. Trypsin is inhibited by organophosphorous compounds such as diisopropylfluorophosphate and porcine-derived inhibitors. Used in protein sequencing and tissue disassociation.
Appearance
Off-white, homogeneous, free flowing powder
Solubility
Light yellow, clear, complete
Manufactured using Trypsin 1:250 (Porcine), Cat# T8670
Source: Porcine pancreas Trypsin: ≥ 250u/mg Chymotrypsin: ≥ 40u/mg Endotoxin (LAL): ≤ 10Eu/ml Porcine Parvovirus (Hemagglutination, PCR): None Detected E. coli: None Detected
Unit Definition
Trypsin: The amount of enzyme which causes an increase in absorbance at 253nm of 0.003 per minute at 25°C
Directions per Liter
Dissolve 10.1g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at 4°C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
| Components in | g/L |
| Dextrose | 1.00 |
| EDTA•Na2•2H2O | 0.20 |
| Phenol Red, Sodium | 0.002 |
| Potassium Chloride | 0.40 |
| Sodium Chloride | 8.00 |
| Trypsin 1:250 | 0.50 |
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