Williams’ Medium E, w/o L-Glutamine, Glucose, Methionine, Cysteine, Cystine, Glycine, Serine, Choline Chloride, Phenol Red

Grade

Cell Culture Grade

EU Commodity Code

38210000

Shipping Temp

Storage Temp

RT/4°C

Media for Mammalian Cell Culture

Appearance

White to off-white, homogeneous, free flowing powder

Solubility

Colorless, clear, complete

As Reported

Endotoxin

≤1EU/ml

Directions per Liter

Dissolve 8.39g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Media Formulation

Components shown as g/liter

Inorganic Salts

Calcium Chloride•2H2O	0.265
Cupric Sulfate•H2O	0.0000001
Ferric Nitrate•9H2O	0.0000001
Magnesium Sulfate	0.0977
Manganese Chloride•4H2O	0.0000001
Potassium Chloride	0.4
Sodium Chloride	6.8
Sodium Phosphate Monobasic	0.122
Zinc Sulfate•7H2O

Amino Acids

L-Alanine	0.09
L-Arginine	0.05
L-Asparagine•H2O	0.02
L-Aspartic Acid	0.03
L-Cysteine (free acid)	Absent
L-Cystine•2HCl	Absent
L-Glutamic Acid	0.0445
L-Glutamine	Absent
Glycine	Absent
L-Histidine	0.015
L-Isoleucine	0.05
L-Leucine	0.075
L-Lysine•HCl	0.08746
L-Methionine	Absent
L-Phenylalanine	0.025
L-Proline	0.03
L-Serine	Absent
L-Threonine	0.04
L-Tryptophan	0.01
L-Tyrosine•2Na•2H2O	0.05045
L-Valine	0.05

Vitamins

Ascorbic Acid•Na	0.00227
D-Biotin	0.0005
Choline Chloride	Absent
Vitamin B-12	0.0002
Calciferol	0.0001
Folic Acid	0.001
myo-Inositol	0.002
Menadione (sodium bisulfate)	0.00001
Niacinamide	0.001
D-Pantothenic Acid•Ca	0.001
Pyridoxal•HCl	0.001
Riboflavin	0.0001
Thiamine•HCl	0.001
DL-a-Tocopherol Phosphate•2Na	0.00001
Retinol Acetate	0.0001

Other

D-Glucose	Absent
Glutathione	0.00005
Methyl Linoleate	0.00003
Phenol Red, Sodium	Absent
Pyruvic Acid, Sodium	0.025

Total:

8.39g/L

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W1105-10 Williams’ Medium E, w/o L-Glutamine, Glucose, Methionine, Cysteine, Cystine, Glycine, Serine, Choline Chloride, Phenol Red (Powder)

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