MF1; RAD2; Maturation Factor-1; DNase IV
Recombinant protein corresponding to Met1~Thr195 from human Flap Structure Specific Endonuclease 1, with a N-terminal His-Tag, expressed in E. coli.
Applications
Suitable for use as a positive control, immunogen, SDS-PAGE and Western Blot. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Form
Supplied as a lyophilized powder in 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to 01-1mg/ml. Do not vortex.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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