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138723 PSMD9, Recombinant, Human, aa1-223, His-Tag (26S Proteasome Non-ATPase Regulatory Subunit 9, p27, Rpn4) CAS:

Specifications
References
Grade
Purified
Molecular Weight
27.1
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

PSMD9 acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process.

Source
Recombinant protein corresponding to aa1-223 from human PSMD9 at N-terminal, fused to His-tag expressed in E.coli.
Molecular Weight
~27.1kD (246aa)
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSDEEAR QSGGSSQAGV VTVSDVQELM RRKEEIEAQI KANYDVLESQ KGIGMNEPLV DCEGYPRSDV DLYQVRTARH NIICLQNDHK AVMKQVEEAL HQLHARDKEK QARDMAEAHK EAMSRKLGQS ESQGPPRAFA KVNSISPGSP ASIAGLQVDD EIVEFGSVNT QNFQSLHNIG SVVQHSEGKP LNVTVIRRGE KHQLRLVPTR WAGKGLLGCN IIPLQR
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥90% (SDS-PAGE)
Concentration
~1mg/ml
Form
Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.15M sodium chloride, 1mM DTT, 10% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Kaneko T., et al. (2009) Cell. 137:914-925 2. Watanabe T.K., et al. (1998) Genomics. 50:241-250.
USBio References
No references available
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