USBio Logo

143424 Leukemia Inhibitory Factor, Recombinant, Human (LIF, Differentiation-stimulating Factor, D Factor, Melanoma-derived LPL Inhibitor, MLPLI, INN=Emfilermin, HILDA) CAS:

Specifications
Grade
Highly Purified
Swiss Prot
P15018
Gene ID
3976
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

LIF is a pleiotrophic factor produced by multiple cell types including T cells, myelomoncytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acutephase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase inadipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant human LIF is a 19.6kD protein containing 180aa residues including three disulfide bonds.

Biological Activity
Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤0.1ng/ml, corresponding to a specific activity of ≥1x10e7 units/mg.
Amino Acid Sequence
SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
Recombinant, E. coli
Form
Supplied as a lyophilized powder from 10mM Sodium Phosphate, pH 7.5. Reconstitute with sterile ddH2O to a concentration of 0.1-1.0mg/ml. Do not vortex.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
  • Contact Us

    Visit our technical library or contact our support staff to answer your questions.

    Telephone:
    1-800-520-3011

    Library | Contact

    Distributors

    For customers outside of the United States, please use one of our many distributors.

    View Distributors

    Payment Methods

    We accept the following payment methods as well as pay-by-invoice.

    MasterCard Visa PayPal
    © 2023-2024 United States Biological - All Rights Reserved