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153382 Adiponectin Receptor 1 (ADIPOR1) Recombinant, Human CAS:

Specifications
Grade
Purified
Swiss Prot
Q96A54
Molecular Weight
47.5
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-70°C

Recombinant protein corresponding to aa1-136 with a N-Terminal His-Tag and GST-Tag from human Adiponectin Receptor 1 expressed in E. coli. Swiss/UniProt Accession: Q96A54.

Molecular Weight
47.5kD
Purity
≥90%
Amino Acid Sequence
MSSHKGSVVA QGNGAPASNR EADTVELAEL GPLLEEKGKR VIANPPKAEE EQTCPVPQEE EEEVRVLTLP LQAHHAMEKM EEFVYKVWEG RWRVIPYDVL PDWLKDNDYL LHGHRPPMPS FRACFKSIFR IHTETG
Predicted Isoelectric Point
6.0
Predicted Molecular Weight
45.4kD
Accurate Molecular Mass
46kD as determined by SDS-PAGE reducing conditions
Subcellular Location
Membrane
Applications
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Source
Recombinant, E. coli
Purity
≥90%
Form
Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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