Source
Recombinant protein corresponding to aa31-97 from human Peptide YY, fused to His-tag and T7-tag at N-terminal, expressed in E. coli (Accession# P10082).
AA Sequence
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- IKPEAPREDA SPEELNRYYA SLRHYLNLVT RQRYGKRDGP DTLLSKTFFP DGEDRPVRSR SEGPDLW
Preditcted Molecular Weight
17.7kD
Subcellular Location
Secreted.
Predicted isoelectric point
8.2
Applications
Suitable for use in ELISA, Western Blot, Immunoprecipitation, SDS-PAGE. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Included Protein Molecular Weight Marker
168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed. MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD Double intensity: 10kD, 18kD, 26kD Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt.
Storage and Stability Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Source
Recombinant, E. coli
Form
Supplied as lyophilized form from 20mM Tris, 500mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose. Reconstitute in sterile PBS, pH 7.3.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.