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171831 BirA, Recombinant, GST-Tag, aa2–321 CAS:

Specifications
References
Grade
Purified
Swiss Prot
P06709
Accession Number
NP_418404
Molecular Weight
61
EU Commodity Code
30021019
Shipping Temp
Dry Ice
Storage Temp
-70°C

Biotin ligase BirA, GenBank Accession No. NP_418404, a.a.2-321 with N-terminal GST-tag, MW = 61kD, expressed in an E. coli cell expression system.

Source
Recombinant protein corresponding to aa2-321 of BirA at the N-terminus, fused to GST tag expressed in E.coli.
Molecular Weight
~61kD
Application
Useful for covalently biotin-labeling of acceptor proteins for detection and purification assays.
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
Source
E. coli
Purity
~78%
Form
Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 16mM glutathione, 3mM DTT, 20% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Li, Y., et al., Protein Expr Purif. 2012 Mar; 82(1): 162-7. 2. Chakravartty, V., et al., J Bacteriol. 2012 Mar; 194(5): 1113-26.
USBio References
No references available
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