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171869 HCV1a (D168V), Recombinant (Hepatitis C virus genotype 1a) CAS:

Specifications
References
Grade
Purified
Swiss Prot
P27958
Accession Number
NC_004102
Molecular Weight
22.4
EU Commodity Code
30021019
Shipping Temp
Dry Ice
Storage Temp
-70°C

Fusion protein corresponding to the serine protease NS3 (a.a.3-181) and cofactor NS4A (a.a.21-32) from Hepatitis C virus genotype 1a (GenBank Accession No. NC_004102) with Asp-to-Val mutation on a.a.168, and N-terminal FLAG-His tag, MW=22.4kD, expressed in an E. coli expression system.

Source
Recombinant protein corresponding to aa3-181 from HCV1a at the N-terminus, with Asp-to-Val mutation on aa 168, an N-terminal FLAG-His-Tag, and a 4aa linker, expressed in E.coli.
Molecular Weight
~22.4kD
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
AA Sequence
MDYKDDDDKHHHHHHGCVVIVGRIVLSGSGSITAYAQQTRGLLGCIITSLTGRDKNQVEGEVQIVSTATQTFLATCINGVCWTVYHGAGTRTIASPKGPVIQMYTNVDQDLVGWPAPQGSRSLTPCTCGSSDLYLVTRHADVIPVRRRGDSRGSLLSPRPISYLKGSSGGPLLCPAGHAVGLFRAAVCTRGVAKAVVFIPVENLETTMRS
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
Source
Recombinant, E. coli
Purity
~53% (SDS-PAGE)
Form
Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 0.04% Tween 20, 250mM imidazole, 20% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. 1. Lin, C., et al., Hepatitis C Viruse. Norfolk (UK): Horizon Bioscience; 2006. Chapter 6. 2. Lang Kuhs, K.A., et al., Mol. Ther. 2012 May;20(3):669-678. 3. Massariol, MJ., et al., Biochem Biophys Res Commun. 2010 Jan 1;391(1):692-697.
USBio References
No references available
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