CD274, Recombinant, FC:Mouse CAS

Grade

Highly Purified

Accession Number

NP_068693.1

EU Commodity Code

30021019

Shipping Temp

Blue Ice

Storage Temp

-20°C

Programmed death ligand 1 (PD-L1, B7-H1 or CD274) is a member of the growing B7 family of immune proteins that provide signals for both stimulating and inhibiting T cell activation. CD274 has been identified as one of two ligands for programmed death 1 (PD-1), a member of the CD28 family of immunoreceptors. CD274 is widely expressed in several organs such as heart, skeletal muscle, placenta and lung, and in lower amounts in thymus, spleen, kidney and liver. CD274 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. CD274 expression is also induced in dendritic cells and keratinocytes after IFN-γ stimulation. CD274 expression is also upregulated in a variety of tumor cell lines. Interaction of CD274 with PD-1 results in inhibition of TCR mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses.. The CD274 - PD-1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.

Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

Recombinant protein corresponding to aa19-237 from the extracellular domain of mouse CD274, fused to the N-terminus of the Fc region of a mutant mouse IgG2a, expressed in CHO cells.

Biological Activity

Shows the biological function of the CD274 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain.

Endotoxin

<0.06EU/ug (LAL test; Lonza)

Storage and Stability

Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Source

Recombinant, CHO cells

Purity

≥98% (SDS-PAGE)

Concentration

~0.1mg/ml (after reconstitution)

Form

Supplied as a liquid in PBS. Reconstitute with sterile PBS.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.