This protein may be used in functional toxicity assays in combination with rLukD, or as a control protein in ELISA assays or Western blotting when detecting toxins produced by different strains of S. aureus.
Purified, Staphylococcus aureus recombinant leukocidin-E (rLukE). The rLukE (tag free) is expressed in E. coli and purified by FPLC. The theoretical molecular weight of the protein is 34,819 Daltons.
Applications
Suitable for use in ELISA, Western Blot and Cytotoxicity assay. Other applications not tested.
Recommended Dilutions
Western Blot: 100ng Optimal dilutions to be determined by the researcher.
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
Staphylococcus aureus
Form
Supplied as a liquid in PBS, 5% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
TDS
NCBI
UniProt
Tech Support
Customer Service
