This protein may be used in functional PVL toxicity assays in combination with LukF-PV, or as a control protein in ELISA assays or Western blotting when detecting LukS in PVL (+) strains of S. aureus.
Purified, tag-free Staphylococcus aureus Panton-Valentine Leukocidin (PVL) S subunit (LukS-PV). The theoretical molecular weight of the protein is 32,465 Daltons.
Applications
Suitable for use in ELISA, Western Blot and Cytotoxicity assay. Other applications not tested.
Recommended Dilutions
Western Blot: 100ng Cytotoxicity assay: 1-100nm Optimal dilutions to be determined by the researcher.
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
Recombinant, E. coli
Form
Supplied as a liquid in PBS, 5% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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