The causative agent of Syphilis is a bacterium: Treponema pallidum. The immune responses to T. pallidum are primarily mediated by the following membrane lipoproteins: TpN47, TpN17, TpN15 and TmpA. TpN47 is highly immunogenic and activates endothelial cells, while TpN17 and TpN15 induce antibody responses.
In independent studies, TmpA is the most frequently identified antigen (95%) while the remaining are recognised with the following prevalence: TpN47, 92.5%; TpN17, 89.5%; and TpN15, 67.5%.
Recombinant protein corresponding to Syphilis TmpA expressed in E. coli.
Activity
Binds Syphilis antibodies
Applications
Suitable for use in ELISA, Western Blot and Lateral Flow. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Concentration
~0.5mg/ml (after reconstitution)
Form
Supplied as a lyophilized powder in 20mM Na-carbonate, pH 10.0. No preservative added.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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