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A0869-56H Adenosine A2a Receptor, Western Blot Positive Control (A2aR, ADORA2A, RDC8) CAS:

Specifications
References
Swiss Prot
P11617
Applications
WB
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C

The purine nucleoside adenosine modulates diverse physiological functions including induction of sedation, vasodilatation, suppression of cardiac rate and contractility, neurotransmitter release, inhibition of platelet aggregation and lipolysis. Adenosine released from cells interacts with membrane receptors (adenosine receptors, ARs). Based upon, biochemical and pharmacological criteria, ARs have been classified into A1, A2a, A2b, and A3. The high affinity receptor A1 inhibits adenyl cyclase, whereas low affinity receptor A2a stimulates the cyclase via G proteins. A2a receptor is believed to cause vasorelaxation in coronary artery. Various, ARs and their subtypes have recently been cloned from several species. ARs belong to the superfamily of G-protein coupled receptors and predicted to contain 7 transmembrane domains. The N-termini are predicted to be extracellular and the C-termini cytoplasmic. There is an overall 30% homology among the four ARs. ARs are distributed throughout the body. Gene location of various ARs: A1 (Chromosome 1; q.31.3-32.2); A2a (Chromosome 22); A2b (Chromosome 17; p.11.2-12); A3 Chromos.1).

Source
A2aR protein, from bovine brain.
Applications
Suitable for use in Western Blot. Not suitable for ELISA or other applications where native protein is required. Other applications not tested.
Recommended Dilution
Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Bovine brain
Concentration
Not determined
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Libert, F. et al (1989) Science 244, 569-572. 2. Tucker, AL and Linden, J (1993) Cardiovascular Res. 27, 62-67. 3. Olah, ME and Stile, G (1995) Ann. Rev. Pharmacol. 35, 581-606. 4. Marala, RB and Mustafa, SJ (1998) J Pharm. Exp. Therap. 286, 1051-1057.
USBio References
No references available
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