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A0869-66G Adenosine A2b Receptor, Human Control Peptide (A2b) CAS:

Specifications
References
Grade
Highly Purified
Specificity
Sequence Homology: The peptide used demonstrates 75% homology (12/16 AA) with rat, 62% (11/16 AA) with mouse A2bR sequences.
Applications
E
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C

A 16 amino acid sequence corresponding to human brain A2bR gene (1). This region corresponds to the 2nd extracellular domain.

Adenosine deaminase (ADA; EC 3.54.4) catalyzes deamination of adenosine to inosine. ADA is found in primarily cytosolic. It has also been located at the cell membrane and implicated in the control of the extracellular concentration of adenosine.
The purine nucleoside adenosine modulates diverse physiological functions including induction of sedation, vasodilatation, suppression of cardiac rate and contractility, neurotransmitter release, inhibition of platelet aggregation and lipolysis. Adenosine released from cells interacts with membrane receptors (adenosine receptors, ARs). Based upon, biochemical and pharmacological criteria, ARs have been classified into A1, A2a, A2b, and A3. The high affinity receptor A1 inhibits adenyl cyclase, whereas low affinity receptor A2a stimulates the cyclase via G proteins. A2a receptor is believed to cause vasorelaxation in coronary artery. Various, ARs and their subtypes have recently been cloned from several species. ARs belong to the superfamily of G-protein coupled receptors and predicted to contain 7 transmembrane domains. The N-termini are predicted to be extracellular and the C-termini cytoplasmic. There is an overall 30% homology among the four ARs. ARs are distributed throughout the body. Gene location of various ARs: A1 (Chromosome 1; q.31.3-32.2); A2a (Chromosome 22); A2b (Chromosome 17; p.11.2-12); A3 (Chromos.1).
Applications
Suitable for use in ELISA, Antibody Blocking. Other applications not tested.
Recommended Dilution
ELISA: 50-100ng A1R control peptide/well. Antibody Blocking: 5-10ug per 1ug A0869-66D (affinity purified antibody) or 1ul A0869-66A (antiserum). Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Human synthetic peptide
Purity
Highly purified
Concentration
~1mg/ml
Form
Supplied as a liquid in PBS, pH 7.4
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Pierce, KD et al (1992) BBRC 187, 86-93. 2. Tucker, AL and Linden, J (1993) Cardiovascular Res. 27, 62-67. 3. Olah, ME and Stile, G (1995) Ann. Rev. Pharmacol. 35, 581-606. 4. Puffinbarger, NK (1995) Mol. Pharmacol. 47, 1126-1132.
USBio References
No references available
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