Source
Neutralized (pI = 6.3), deglycosylated avidin purified from egg white.
Mr/Structure
60kD tetramer of four identical subunits, each containing a single binding site.
Applications
• Flow cytometry • FLISA • Immunoblotting • Immunohistochemistry
Recommended Dilution
Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 6 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Form
Supplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Conjugated to APC.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.