Bone Morphogenetic Protein 2, Recombinant, Human CAS

Grade

Highly Purified

Swiss Prot

P12643

Molecular Weight

EU Commodity Code

30021019

Shipping Temp

Storage Temp

-20°C

BMPs (Bone Morphogenetic Proteins) belong to the TGF-β superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 plays an important role in cardiac morphogenesis, and is expressed in a variety of tissues, including lung, spleen, brain, liver, prostate, ovary, and small intestine. The functional form of BMP-2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. Each BMP-2 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 23 amino acid signal sequence for secretion, and a 259 amino acid propeptide. After dimerization of this precursor, the covalent bonds between the propeptide (which is also a disulfide-linked homodimer) and the mature BMP-2 ligand are cleaved by a furin-type protease. Recombinant Human/Murine/Rat BMP-2 is a 26kD homodimeric protein consisting of two 115 amino acid polypeptide chains.

Recombinant protein corresponding to human Bone Morphogenetic Protein-2, expressed in E. coli.

Amino Acid Sequence

MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR

Biological Activity

Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.5-1ug/ml.

Storage and Stability

Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. RReconstitute with sterile 100mM acetic acid. Add 0.1% HSA or BSA for long term storage. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Source

Recombinant, E. coli

Form

Supplied as a lyophilized powder from 10mM sodium citrate, pH 3.5. Reconstitute with sterile 100mM acetic acid to ≥0.1mg/ml. Add 0.1% HSA or BSA for long term storage.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

References

1. Reppe, S., et al., Biochem. Biophys. Res. Commun. 324(1): 218-223 (2004). 2. Kim, Y.J., et al., J. Biol. Chem. 279(49): 50,773-50,780 (2004). 3. Seeherman, H.J., et al., J. Bone Joint Surg. Am. 86-A(9): 1961-1972 (2004). 4. Kaden, J.J., et al., J. Heart Valve Dis. 13(4): 560-566 (2004). 5. Mummaneni, P.V., et al., J. Neurosurg. Spine 1(1): 19-23 (2004). 6. Wajih, N., et al., J. Biol. Chem. 279(41): 43,052-43,060 (2004).

USBio References

1. Grünhagen J., et al. MiR-497∼195 cluster microRNAs regulate osteoblast differentiation by targeting BMP signaling. 2015. J Bone Miner Res. 30(5):796-808. doi: 10.1002/jbmr.2412.

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B2553-06A Bone Morphogenetic Protein 2, Recombinant, Human (BMP2) CAS:

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