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C2425-01R CD70 Human, CD8 Mouse Fusion Protein (Biotin) CAS:

Specifications
References
Grade
Affinity Purified
Conjugate
Biotin
Applications
E FC
Molecular Weight
34.8
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Human CD70 (TNFSF7) is the ligand for CD27 (TNFRSF7) and is notably absent on resting lymphocytes. CD70 is expressed after activation on about 20% of T cells and on about 70% of B cells. Recombinant CD70-muCD8 binds to cell surface CD27 on human peripheral blood leukocytes.

Molecular Structure:  A soluble molecule consisting of the extracellular domain (167aa) of murine CD8 alpha fused to 146 (c-term) amino acids of the extracellular domain of human CD70, with a predicted monomeric weight of 34.8kD. Extracellular mature murine CD8alpha: (28)KPQAPEL....GLDFACD(194); Linking amino acids: GT; extracellular human CD70: (48)LPLESL....VQWVRP(193).
Applications
Suitable for use in ELISA and Flow Cytometry. Other applications not tested.
Recommended Dilution Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Source
CHO cells
Purity
Purified by affinity chromatography.
Concentration
~0.25mg/ml
Form
Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. R.Q. Hintzen, et al, (1994) J Immunol 152: 1762-1773. 2. M.R. Bowman, et al, (1994) J Immunol 152: 1756-1761. 3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1137-1138. 4. K. Agematsu, et al, (1995) J Immunol 154: 3627-3635.
USBio References
No references available
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