Clostridium difficile Toxoid A is a highly purified, non-toxic preparation derived from C. difficile toxin A. It has been developed either for use as an immunogen to raise antibodies or for use in clinical diagnostic assays. Formaldehyde is use to convert the toxin into Toxoid A and quality control data illustrates that this treatment has no significant effect on the antigenicity of the resultant preparation.
Native toxin A, strain VPI10463 from C. difficile, converted to inactive toxoid form
Inactivation
Inactivated by formaldehyde. No cell rounding in the sensitive verocytotoxicity assay at 2ug/ml compared to active toxin lethal dose of 0.2ng/ml.
Applications
Suitable for use in Direct ELISA. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note
No test guarantees a product to be non-infectious. All materials should be handled as if potentially infectious. Generally accepted laboratory practices appropriate for infectious materials should be employed when handling this product.
Form
Supplied as a liquid in 0.05M Hepes, 0.15M sodium chloride, 5% sucrose
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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