EC=3.4.24.3.
Collagenases degrade native helical collagen fibrils.The substrate, collagen, is the major fibrous component of animal extracellular connective tissue: skin, tendon, blood vessels, bone, etc. Suitable for use in cell dissociation procedures.
Activity
≥125 units/mg dry weight. Caseinase: As reported Clostripain: As reported Tryptic: As reported
Unit Definition
1 unit releases 1umole of L-leucine equivalents from collagen in 5 hours at 37ºC, pH 7.5.
Absorbance (A280)
As Reported
Collagenase nomenclature
Type 1: Crude collagenase has the original balance of collagenase, caseinase, clostripain and tryptic activities. Type 2: Contains higher relative levels of protease activity, particularly clostripain Type 3: Contains lowest levels of secondary proteases Type 4: Designed to be especially low in tryptic activity to limit damage to membrane proteins
Storage and Stability
Lyophilized powder may be stored at 4°C. Stable for 6 months after receipt at 4°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Source
Clostridium histolyticum
Form
Supplied as a lyophilized powder
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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