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E3453-20C Erythrina cristagalli (ECA) (AP) CAS:

Specifications
References
Grade
Purified
Conjugate
AP
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Cockspur Coral Tree

Pure Erythrina cristagalli lectin (ECA) from coral tree, Alkaline Phosphatase conjugated.

Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms.
Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.
Carbohydrate Specificity: Galactose (beta1,4) N-Acetylglucosamine.
Inhibitory Carbohydrate: N-Acetyllactosamine > Lactose > N-Acetylgalactosamine > Galactose.
Activity: 1-2ug/ml will agglutinate neuraminidase treated human erythrocytes.
Chemical Used for Conjugation: Alkaline Phosphatase.
Storage and Stability
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Purity
Purified
Concentration
As reported
Form
Supplied as a liquid in 0.02M Sodium Bicarbonate, pH 9.0-9.5, 20-50% glycerol. Labeled with Alkaline Phosphatase (AP).
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Lis, H. and Sharon, N. (1987). Meth. Enzymol. 138:544, 2. Lis, H. et al. (1985). Phytochemistry. 24: 2803, 3. Crowley, J.F. and Goldstein, I.J. (1981).FEBS. Lett. 130: 149-152, 4. Webb, C.W., et al. (1985). In “Cell Membranes and Cancer”. T. Galeotti, et al. Eds. Page 13. Elsevier, Amsterdam.
USBio References
No references available
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