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F0018-32 Factor XIa Substrate (D-LPR-ANSNH-C3H7•2HCl) CAS:

Specifications
References
Molecular Weight
721.74
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C

Substrates containing the fluorescent reporter group 6-amino-1-naphthalene-sulfonamide (ANSN) are useful compounds for monitoring the enzyme activity of various serine proteases (1-12). In this class of compounds, the ANSN reporter group is linked (in the R1 position) to short tri-peptide sequences. The peptide sequences are designed to optimize the interaction between the enzyme and substrate. Additional components which may be added to the R2 and R3 positions reflect changes in the P' subsite positions, and generally affect the kinetic parameters of the substrates. Compounds which are effective substrates are hydrolyzed between the tri-peptide and the ANSN group. Once cleaved from the peptide moiety, the ANSN group exhibits about a 1000 fold increase in relative fluorescence.

The kinetic properties listed below will aid in the selection of an appropriate substrate. The ANSN substrates have proved especially useful for the analyses of factor VIIa (1-5,7,9,10,12). Although the substrate hydrolysis rates are relatively slow for factor VIIa alone, several compounds exhibit a large change in kcat when tissue factor is incorporated into the assay system.
Emission Wavelength
470nm
Excitation Wavelength
352nm
Light artifacts
Minimized by using A390-450nm long-pass cutoff filter in the emission beam.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Light sensitive.
Concentration
~5mg/ml
Form
Supplied as a liquid in DMSO.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Butenas, S., et al., Biochemistry, 31, 5399 (1992). 2. Lawson, J.H., et al., J. Biol. Chem., 267, 4834 (1992). 3. Lawson, J.H., et al., Methods in Enzymology, 222, 177 (1993). 4. Butenas, S., et al., Biochemistry, 32, 6531 (1993). 5. Butenas, S., et al., J. Biol. Chem., 269, 25838 (1994). 6. Butenas, S., et al., Anal. Biochem., 225, 231 (1995). 7. Butenas, S., and Mann, K.G., Biochemistry, 35, 1904 (1996). 8. Butenas, S., et al., Biochemistry, 36, 2123 (1997). 9. Butenas, S., et al., J. Biol. Chem., 272, 21527 (1997).  10. Butenas, S., et al., Thromb. Haemost., 78, 1193 (1997). 11. Hockin,M.F., et al., Arterioscler. Thromb. Vasc. Biol., in press (1997). 12. Butenas, S., et al., U.S.A. patent # 5,399,487.
USBio References
No references available
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