Recombinant corresponding to bovine GST A1-1 expressed in E. coli.
Molecular Weight
~23kD
Biological Activity
~4 Units/mg using spectrophotometric determination of 1- chloro-2,4-dinitrobenzene (CDNB) conjugation with reduced glutathione (1mM) in 100mM NaPO4 (pH 6.5) at room temperature.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥95% (SDS-PAGE)
Concentration
As Reported
Form
Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 50mM sodium chloride, 1mM DTT, 1mM EDTA, 50% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
Raffalli-Mathieu, F., et. al; (2007) Biochimica et Biophysica Acta 1770:130-136