In a 50ul reaction, assay HDAC activity using HDAC (enzyme, lysate, etc.), substrate, HDAC assay buffer, and test inhibitor. Incubate microtiter plate at 37°C for 30 minutes. Add an equal volume (50ul) of HDAC Assay Developer to each well. Incubate the plate at room temperature for 15 minutes. Read sample in a microtiter-plate reading fluorimeter capable of excitation at a wavelength in the range of 350-380nm and detection of emitted light in the range of 440-460nm. “Blank” value (no enzyme negative control) is subtracted from all other values.

Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

≥ 95% by HPLC

As reported

Supplied as a liquid in TBS.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.